Fig. 6: AtPRMT3-RPS2 balances the trade-off between a general growth and stress-responsive state.

a, b Venn diagrams illustrating down-regulated (a) and up-regulated (b) Translational Efficiency (TE). c, d The heatmaps showing up-regulated and down-regulated TE. Heatmap (c) was genes with down-regulated TE in atprmt3-2 and rps2a2b-1 excluding rps2c-1 (724 in a). Heatmap (d) was genes with up-regulated TE in atprmt3-2 and rps2a2b-1 excluding rps2c-1 (648 in b). The heatmap encompasses eight groups based on the patterns of their translational status, with representative enriched Gene Ontology (GO) terms shown. The log₂(TE) is shown by the color scale. e Representative genome browser view of RNA-seq and Ribo-seq for CHS. f Expression validation for CHS. Total RNA and polysome RNA from Col-0, atprmt3-2, rps2a2b-1, and rps2c-1 mutants were used for qRT-PCR. ACTIN2 was used as a control. Data are presented as mean values ± SD (n = 3). g Translation efficiency analysis in (f). Data are presented as mean values ± SD (n = 3). h Induction of CHS expression by cold stress. Twelve-day-old seedlings of Col-0 were treated at 4 °C for 0 and 24 h, and then used for qRT-PCR. ACTIN2 was used as a control. Data are presented as mean values ± SD (n = 4). i Freezing stress phenotype. Twelve-day-old seedlings of Col-0, atprmt3-2, chs and atprmt3chs were subjected to freezing treatment at −6 °C for 1 h for non-acclimated (NA) condition. j The survival rates of plants in (i). Data are presented as mean values ± SD (n = 4). (f, g, j) P value was calculated by One-way ANOVA with Dunnett’s multiple comparisons test. (h) P value was calculated by two-sided unpaired Student’s t test. Source data are provided as a Source Data file.