Fig. 4: Voltage dependency of AtHAK5.

A Normalized whole-oocyte currents of AtHAK5 and CIPK23/CBL1 co-expressing oocytes. K⁺-induced peak currents (ΔI_peak) at different voltages (as indicated in the figure) (pH4.5) were plotted against the applied K⁺-concentration. Data points were fitted with a Michaelis-Menten equation. (n = 4 experiments, mean ± SEM). B Maximum currents (I_max) derived from the Michaelis-Menten fits shown in A) were plotted against the applied voltage (n = 4 experiments, mean ± SEM). C) K_m (K⁺) values derived from the Michaelis-Menten fits shown in A) were plotted as a function of the applied voltage (n = 4 experiments, mean ± SEM). D Normalized whole-oocyte currents of AtHAK5 and CIPK23/CBL1 co-expressing oocytes. Peak currents (ΔIpeak) were induced with 200 µM K⁺ at different voltages (as indicated in the figure) and plotted against the applied H⁺-concentration. Data points were fitted with a Hill equation (n = 5 experiments for −150 mV and n = 7 experiments for −60, −90, and −120 mV, mean ± SD). E) K_m [H⁺] values derived from the data shown in D) were plotted against the applied voltage (mean ± SD).