Fig. 3: Dynein recruitment by kinetochore-specific adapters is required to orient chromosomes in the kinetochore dynein module-only state.

a Consequences of removal of the RZZ complex subunit ROD-1 in the condition used to generate the kinetochore dynein module-only state. Representative images with graphs plotting chromosome angles relative to the spindle axis, measured as in Fig. 1e, are shown. The kinetochore dynein module-only graph is the same as in Fig. 2c to aid comparison. Scale bars, 2.5 µm. b Comparison of SPDL-1 WT to the SPDL-1 mutant (F199 > A in the conserved Spindly motif) that perturbs dynein recruitment in the kinetochore dynein module-only state. Cartoons above depict the compared conditions; note that there was no transgene-encoded CH^mut NDC80 present. Representative images and graphs plotting chromosome angles relative to the spindle axis, measured as in Fig. 1e, are shown. Scale bars are 5 µm (full spindle view) and 2.5 µm (magnified region). c Images of in situ-tagged KNL-1::GFP and mCherry::H2b in the kinetochore dynein module-only state highlighting biorientation. Scale bar, 2 µm. d Images of in situ-tagged KNL-1::GFP and GFP::H2b in the kinetochore dynein module-only state highlighting poleward sister separation, despite lack of congression. The first frame was arbitrarily set to 0 s. Scale bars, 2 µm and 1 µm (KNL-1::GFP and GFP::H2b time series, respectively). n is the number of embryos analyzed. Source data are provided as a Source Data file.