Fig. 1: Piezo1 activation inhibits brain functional hyperemia (FH).

a Whisker stimulation experimental scheme (left) and representative traces showing whisker stimulation-induced changes in CBF (∆CBF) in the contralateral somatosensory barrel cortex of a C57BL/6J mouse before and after Yoda1 (30 µM) application onto a cranial window. b Time course reflects the change in FH amplitude (∆CBF) from the experiment in a after the application of Yoda1. Each circle denotes one stimulation. c Average waveforms of whisker stimulation-induced CBF changes in the absence and presence of Yoda1 (n = 5 mice). d Summary data showing the effect of Yoda1 on CBF increases (n = 5 mice). e Baseline CBF in arbitrary units (arb. units) before and after Yoda1 application. f Impact of cortical application of Yoda1 on mean arterial blood pressure (MAP) measured using a femoral artery catheter (n = 5 mice). g, h Vehicle application had no effect on CBF increases or MAP (n = 4 mice). i Two-photon laser scanning microscopy experimental scheme and images of penetrating arteriolar diameter before (dotted) and after (solid) whisker-stimulation evoked arteriolar dilation. Scale bar = 5 µm. In a control mouse, whisker stimulation evoked a large increase in arteriolar diameter that was absent in the presence of Yoda1 (cortical, 30 µM). The experiment was repeated independently in 5 C57BL/6J mice (summarized in k). (j) Representative traces of arteriolar dilation in response to a 30 s whisker stimulation before and after Yoda1. k Summary of arteriolar responses to the described stimuli (n = 5 mice). Wilcoxon statistical test was used in d-h (one-sided in d and two-sided in f–h) and paired Student’s t-test (two-sided) in k (*P < 0.05). All error bars are standard error of the mean (SEM). Data in c, d, f, g, h, k are presented as mean values ± SEM. Source data are provided as a Source Data file.