Fig. 6: Endothelial Piezo1 gain-of-function mice display a memory impairment phenotype.

a Novel object recognition test in control and Piezo1^cx/cx;Cdh5-Cre+ mice. Heat maps demonstrate the cumulative time spent with familiar (F) and novel (N) objects. b Scatter plot shows the discrimination index (DI%-calculated based on duration) for the two groups (n = 7 control, 9 Piezo1^cx/cx;Cdh5-Cre+ mice). DI of 50% (dotted) indicates equal time spent with novel and familiar objects. c Velocities of control (n = 7) and Piezo1^cx/cx;Cdh5-Cre+ mice (n = 9) during the NOR test. d, e Similar to a and b, test results from Piezo1^cx/cx;Slco1c1-Cre+ and their controls (n = 10 each). f Motor activity of Piezo1^cx/cx;Slco1c1-Cre+ mice and their controls (n = 10 each) during the NOR test. g Percent of alternation over all 6 trials during the spontaneous alternation T-maze was calculated as [(number of correct alternations/6)*100)] in Piezo1^cx/cx;Slco1c1-Cre+ and their controls (n = 11 each). The dotted line represents the 50% chance level. h Choice latency in Piezo1^cx/cx;Slco1c1-Cre+ and control mice over trials 1 to 6. Asterisks highlight significantly higher choice latency in Piezo1^cx/cx;Slco1c1-Cre+ mice at T4, T5 and T6. Data from individual mice are shown, and bold lines and transparent shades are means and SEM, respectively (n = 11 mice each). Statistical tests were unpaired Student’s t test (two-sided, b, c, e, f, g) and two-way ANOVA test in h (*P < 0.05, **P < 0.01, ***P < 0.001). All error bars are SEM. Data in b, c, e-g are presented as mean ± SEM. Source data are provided as a Source Data file.