Fig. 4: Identification of SPMT-dynein complex subunits in ookinetes. | Nature Communications

Fig. 4: Identification of SPMT-dynein complex subunits in ookinetes.

From: A subpellicular microtubule dynein transport machinery regulates ookinete morphogenesis for mosquito transmission of Plasmodium yoelii

Fig. 4

A Schematic of the SPMT-dynein complex, composed of six subunits including DHC, LIC, IC, and LCs (Robl, LC8, and Tctex). B Information of five genes encoding dynein subunits in the genome of P. yoelii. C Two-colored IFA of DHC3 with LIC, IC, Robl, LC8, and Tctex in the ookinetes. Each endogenous protein was C-terminally fused with a 6HA in the 4Myc::dhc3 parasite and five modified lines were generated. Parasites were co-stained with antibodies against HA and Myc. Three independent experiments. Scale bars: 5 μm. D U-ExM of SPMTs and five dynein subunits in the ookinete. Parasites were co-stained with antibodies against α/β-Tubulin and HA. White arrow indicates the ATR. Three independent experiments. Scale bars: 5 μm.

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