Fig. 5: Csr-regulated Two-input and higher order logic gates.

A Fluorescence of Csr-regulated OR Gate (cOR) two hours post-induction. B Fluorescence of Csr-regulated NOR Gate (cNOR) two hours post-induction. C Fluorescence of Csr-regulated AND Gate (cAND) two hours post-induction. D Fluorescence of Csr-regulated NANR Gate (cNAND) two hours post-induction. E Genetic circuit diagram of cAND inside the cOR system. The cAND system is activated when CsrB is expressed and in the presence of salicylic acid. F Fluorescence of cOR in nested cOR-cAND system. G Fluorescence of cAND in nested system. H Genetic circuit diagram of Csr-regulated genetic pulse. I Time course of genetic pulse system under different induction conditions. Samples were grown in biological triplicate, and data presented are the mean values +/- the standard deviation, represented as the error bars.