Fig. 6: cBUFFER recapitulated in multiple industrially relevant bacteria. | Nature Communications

Fig. 6: cBUFFER recapitulated in multiple industrially relevant bacteria.

From: Rewiring native post-transcriptional global regulators to achieve designer, multi-layered genetic circuits

Fig. 6

a Response function of Csr Buffer Gate in MR−1 at different IPTG induction concentrations. b Secondary structure of the 5’ UTR used in the Buffer Gate with each GGA binding site identified in tan. c Fold-Turn on of cBUFFER using the original 5’ UTR sequence, as well as 5’ UTRs with sequentially mutated CsrA binding sites. d Phylogenic tree of bacteria tested that contain homologous Csr/Rsm post-transcriptional networks. e Fold-turn of cBUFFER in each organism. Statistical significance was determined using two-tailed heteroscedastic t-tests between the induced and uninduced samples, in which the p-value < 0.05 (p-values for MR-1 = 0.001, EcN = 0.04, PY79 = 0.01). Samples were grown in biological triplicate, and data presented are the mean values +/- the standard deviation, represented as the error bars.

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