Fig. 1: Experimental setup and quality control of the lipidomic analysis of cells infected with different human pathogenic orthoflaviviruses.

a Huh7 cells were seeded 1 day prior to infection with the different orthoflaviviruses (ZIKV MOI 1, WNV MOI 0.01, TBEV MOI 1, DENV MOI 0.5, and YFV-17D MOI 0.005). 12, 24, and 48 h post infection (hpi) samples were taken for protein and RNA extraction, and fixed for immunofluorescence analysis. b Virus genome equivalents (GE) per µg total cellular RNA normalized to 18S rRNA measured via RT-qPCR (mean ± SEM, n = 3 independent experiments). c Cells were stained with antibodies against orthoflavivirus E protein (magenta), and BODIPY493/503 (green) and Hoechst (blue) were used to visualize lipid droplets (LDs) and nuclei, respectively. Scale bar 10 µm. d Quantification of lipid droplets from more than 45 individual cells from three independent experiments (median ± 95% CI, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, unpaired two-tailed Mann–Whitney U test). e Samples were analyzed by immunoblotting with orthoflavivirus E protein and GAPDH antibodies (shown is one representative experiment of three independent experiments). Source data are provided as a Source Data file.