Fig. 3: Shared oligoclonal TCRαβ clonotypes on cytotoxic CD8⁺ Tconv in affected skin and blister fluid are absent from or unexpanded in unaffected skin.

a UMAPs for paired unaffected and affected skin and blister fluid samples from a single patient with SJS/TEN. b Differential gene expression (two-tailed Wilcoxon, Hochberg adj, p < 0.05) between keratinocytes or CD8⁺ Tconv cells from unaffected and affected skin (n = 1 patient with time-paired samples; unaffected skin keratinocytes, n = 331 cells; unaffected skin CD8⁺ Tconv, n = 142 cells; affected skin keratinocytes, n = 108 cells; affected skin CD8⁺ Tconv, 356 cells). Genes colored red are significantly (p < 0.05) increased (light red < 0.6 log2 fold change (log2FC), dark red > 0.6log2FC). The top 10 genes are labeled. c Top functional TCR CDR3αβ pairings and counts (Ct.) in CD8⁺ Tconv cells. The same top TCR clonotypes across affected samples are highlighted in gray. d Signatures of dominant TCR⁺ CD8⁺ Tconv align to the cytotoxic Tconv cluster. Expression of the top 3 dominantly-expanded TCR (black) on CD8⁺ Tconv cells of each sample. The number of dominant TCR⁺ cells aligned to CD8⁺ Tconv cluster 2 (control) and cluster 3 (cytotoxic) are indicated numerically. e Differential gene expression (two-tailed Wilcoxon, Hochberg adj, p < 0.05) between CD8⁺ Tconv expressing dominant TCRs or other TCRs in affected skin or blister fluids (n = 1 patient with time-paired samples; affected skin DomTCR^NEG, 192 cells; affected skin DomTCR^POS, n = 47 cells; blister fluid DomTCR^NEG, 2441 cells; blister fluid DomTCR^POS, n = 559 cells). Genes colored red are significantly (p < 0.05) increased (light red < 0.6log2FC, dark red > 0.6log2FC). The top 10 genes are labeled. Figure created using Visual Genomics Analysis Studio (VGAS)⁸⁹. Source data are provided as a Source Data file. TCR, T cell receptor; Dom TCR, Dominantly-expanded TCR; MSC, Mesenchymal stromal cell; HSC, Hematopoietic stem cell; NK, Natural killer; DC, Dendritic cell; Tconv, T conventional cell; CDR3, Complementary determining region 3; Ct., count; FDR, false discovery rate; FC, fold change; Dom TCR, dominant TCRs; SJS/TEN, Stevens-Johnson syndrome and toxic epidermal necrolysis.