Fig. 2: Alpha and beta diversity by trial arm.

A Violin plot presenting taxonomic alpha diversity—measured by Shannon and inverse Simpson indices—by trial arm immediately post-intervention (16S rRNA data). Each dot represents an individual sample, grouped by trial arm. Group differences were calculated using pair-wise ANOVA for each diversity measure. Center lines denote median value, with rectangles showing 25–75th percentiles. The violin outlines the distribution of the data, with wider sections representing a higher probability that samples in the dataset will have the corresponding value and narrower sections representing a lower probability. B Principal coordinates analysis plot illustrating beta diversity of samples by trial arm post-intervention and measured by Bray-Curtis dissimilarity, with permutational analysis of variance (PERMANOVA) used as the statistical test (p = 0.841) (16S rRNA data). Each dot represents an individual sample, grouped by trial arm. (n = 276 for placebo, n = 254 for iron and n = 262 for MNPs).