Fig. 4: Osteopontin induces macrophage migration and synergistically polarizes macrophages towards a pro-tumorigenic state with IL-4.
From: Osteopontin is a therapeutic target that drives breast cancer recurrence
a Schematic representation of mouse bone marrow-derived macrophages (BMDMs). Created in BioRender. Muller, W. (2024) BioRender.com/w64j858. b Schematic representation of experimental design for migration assay of BMDM in vitro with various concentrations of recombinant mouse OPN (rmOPN). Created in BioRender. Muller, W. (2024) BioRender.com/h82t760. c Cell count of BMDM cells (n = 3 cell lines) in transwell migration assay towards saline, rmOPN at 1 and 5 μg/mL for 4, 8, and 24 h. Each data point is an average of four counts of four fields of view. d FACS sorting for mouse BMDM cells (n = 3 cell lines) treated with saline, rmOPN, IL-4, and rmOPN and IL-4. Total F4/80+ macrophages from live cells sorted based on the expression of IL-4 receptor (IL-4R) and arginase 1. e Quantification of the percentage of arginase 1high cells out of total F4/80+ macrophages (n = 3 cell lines). f Quantification of the percentage of arginase 1high IL-4Rhigh cells out of total F4/80+ macrophages (n = 3 cell lines). Mean ± SEM for data calculated using Ordinary One Way ANOVA with Tukey’s post hoc test. Each data point is representative of one mouse BMDM cell line for (e, f). Source data are provided as a Source Data file.
