Fig. 2: Myeloid cell subpopulations expressing anti-inflammatory genes were increased by αIL-23p19 administration in NDA mouse lungs.

a Uniform manifold approximation and projection (UMAP) plot showing 11 clusters of CD45⁺ immune cells. b Dot plot showing relative expression of known marker genes of immune cells (x-axis) across all clusters (y-axis) (Supplementary Data 3). The color and diameter of the dots indicate the average expression of the indicated gene and the proportion of cells expressing the gene in each cluster, respectively. c Relative proportions of cells originated from three conditions in the five myeloid cell clusters. Proportions per cluster in each sample were normalized for their sum to be one. d UMAP plots showing subclusters of the indicated myeloid cell clusters. e Relative proportions of cells originated from three conditions in the subclusters of the indicated myeloid cell clusters. Red highlighted clusters with higher proportions of the cells in OVA + LPS/OVA + αIL-23p19 (P19) and OVA + LPS/PBS (PBS) than those in OVA + LPS/OVA (OVA). Proportions per cluster in each sample were normalized for their sum to be one. f Heat map showing gene ontology biological processes (GOBPs) enriched by marker genes for the five selected subclusters and their enrichment significance (p) from the DAVID software as –log₁₀(p-value). Red labeled, GOBPs related to neutrophilic inflammation and subclusters in which those GOBPs are enriched. g Bar plot showing the mean percentage of cells expressing the indicated anti-inflammatory genes whose proteins are localized on the plasma membrane in TREM2^high CM and Circulating NL. Red labeled, candidate genes associated with the inhibitory effects of αIL-23p19. (a–g) n = 3 samples. Data are shown as mean ± s.e.m. Significance was determined by two-way ANOVA with Tukey’s post hoc correction (e).