Fig. 4: CD39 and CD9 are responsible for αIL-23p19-dependent suppression of Th17 cell activation and neutrophilic inflammation in NDA mice. | Nature Communications

Fig. 4: CD39 and CD9 are responsible for αIL-23p19-dependent suppression of Th17 cell activation and neutrophilic inflammation in NDA mice.

From: Distinctive CD39+CD9+ lung interstitial macrophages suppress IL-23/Th17-mediated neutrophilic asthma by inhibiting NETosis

Fig. 4

a Percentages of CD39+CD9+ IMs and (b) Ly6G+ neutrophils in CD45+CD11b-CD11c- cells of lung (n = 7 per group). c Total cell, (d) neutrophil counts in 7 groups: (i) OVA + LPS/PBS (n = 6), (ii) OVA + LPS/OVA (n = 6), (iii) OVA + LPS/OVA + POM1 (n = 6), (iv) OVA + LPS/OVA + αCD9 (n = 5), (v) OVA + LPS/OVA + αIL-23p19 (n = 6), (vi) OVA + LPS/OVA + αIL-23p19 + POM1 (n = 6), and (vii) OVA + LPS/OVA + αIL-23p19 + αCD9 (n = 6). e IL-17 and IL-22 levels in BALF (n = 5 per group). f Representative images of H&E staining and (g) inflammatory scores quantified from H&E staining (n = 4 per group). Scale bar = 100 μm. h Penh values were measured using whole-body plethysmography (n = 6 per group). Data are shown as mean ± s.e.m. Significance was determined by one-way ANOVA with Tukey’s post hoc correction (a–e, g) and two-way ANOVA with Sidak’s post hoc correction (h).

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