Fig. 7: ISCV of RMn-NB-sensitized RT.

a Primary and (b) distant tumor growth curves of CT26 colorectal bilateral tumor-bearing mice treated with Saline, Saline + RT, RMn-NBs + RT, RMn-NBs + RT + αCD8a and RMn-NBs + RT + αPD-L1. The doses of Mn²⁺ and RANa are 5.5 mg kg⁻¹ and 30.5 mg kg⁻¹, respectively. X-ray irradiation is performed after intravenous injection of RMn-NBs (10 mM × 0.2 mL) for 6 h. Treatments are performed with 3 fractions (total 6 Gy) on days 0, 3, and 6, and only primary tumors receive RT (black arrow). αPD-L1 (10 mg kg⁻¹) and αCD8a (10 mg kg⁻¹) are intraperitoneally injected respectively after RT for 6 h, and treatments are performed on days 0, 3, 6, and 9 (red arrow, n = 6 mice). c Primary and (d) distant tumor weights from mice with various treatments (n = 6 mice). The percentages of CD3⁺ CD4⁺ T cells infiltrated within (e) primary and (f) distant tumor tissues detected by FCM (n = 6 mice). The percentages of CD3⁺ CD8⁺ T cells infiltrated within (g) primary and (h) distant tumor tissues detected by FCM (n = 6 mice). The percentages of FoxP3⁺ T cells (Tregs) among CD3⁺ CD4⁺ T cells infiltrated within (i) primary and (j) distant tumor tissues detected by FCM (n = 6 mice). Relative content of IFN-γ in the (k) primary and (l) distant tumor tissues detected by ELISA kit (n = 6 mice). All data are shown as mean ± SD. Statistical analysis is performed using two-tailed Student’s t-test for comparing two groups, and one-way ANOVA analysis of variance for multiple groups. p > 0.05 represent nonsignificance, while p < 0.05 indicate statistical significance. Source data are provided as a Source Data file.