Fig. 5: Wildtype β-Syn alleviates while clinical β-Syn mutants exacerbate α-Syn-induced neurodegeneration.
From: β-synuclein regulates the phase transitions and amyloid conversion of α-synuclein
a Schematic diagram of microinjection in C. elegans. b Representative confocal images of the heads of different C. elegans strains. Scale bar, 100 μm. Zoomed images show enlarged puncta in different strains. Scale bar, 10 μm. α-Syn fused with YFP gives green fluorescence, wildtype and mutant β-Syn fused with mNeptune2.5 shows red fluorescence. c Normalized FRAP recovery curves of α-Syn inclusions in different strains, n = 6 inclusions per group. d Thrashing assay results for different C. elegans strains. Experiments were repeated 3 times, 10 worms were used for each condition. **PThrashing (NL5901 vs β-Syn) < 0.0001, **PThrashing (NL5901 vs α-Syn;β-Syn) < 0.0001, **PThrashing (NL5901 vs α-Syn;V70M) = 0.0009, *PThrashing (NL5901 vs α-Syn;P123H) = 0.0128. e Survival curves (top) and median lifespan (bottom) of N2, N2;β-Syn, N2;V70M and N2;P123H strains. The median lifespan is given as the length in days at which each group reached 50% survival. Experiments were repeated at least 3 times, 20 worms were used for each condition. *Pmedian lifespan (N2 vs β-Syn) = 0.0487, *Pmedian lifespan (N2 vs V70M) = 0.0244, **P median lifespan (N2 vs P123H) = 0.0035. f Survival curves (top) and median lifespan (bottom) of NL5901, α-Syn;β-Syn, α-Syn;V70M and α-Syn;P123H strains. Experiments were repeated at least 3 times, 20 worms were used for each condition. **Pmedian lifespan (NL5901 vs α-Syn;β-Syn) = 0.0034, **Pmedian lifespan (NL5901 vs α-Syn;V70M) = 0.0016, *Pmedian lifespan (NL5901 vs α-Syn;P123H) = 0.0135. Data are presented as mean ± SD, one-way ANOVA was used for multiple experimental groups without adjustment. *P < 0.05; **P < 0.01.. b–f At least three independent experiments were performed with similar results. Source data are provided as a Source Data file.
