Fig. 6: Identification and characterization of Th1-like cells in the liver of PBC mice. | Nature Communications

Fig. 6: Identification and characterization of Th1-like cells in the liver of PBC mice.

From: Single-cell RNA sequencing reveals the pro-inflammatory roles of liver-resident Th1-like cells in primary biliary cholangitis

Fig. 6

a Schematic of the PBC mouse model generated using 2OA-BSA. Figure 6a created in BioRender. Gu, T. (2024) BioRender.com/b84j502. b Representative immunohistochemical images showing distributions of CD4+ and CD8+ T cells in the liver from ctr and 2OA-BSA groups. ctrl indicated control group. All experiments are repeated 5 times for each group. c UMAP showing annotated cell subsets. d UMAP showing the distribution of cells from control and 2OA-BSA groups. e Expression of pro-inflammatory related genes in each cell subset from control and 2OA-BSA groups. f UMAP showing the distribution of annotated T cell subsets from control and 2OA-BSA groups. g Bar plots showing the cell ratio of T cell subsets in different samples and groups. The color represents the T cell subpopulation shown in Fig. 6f. h UMAP showing the expression of Sell, Prdm1, Cd69, Cxcr6. i UMAP showing the expression of Ifng, Jak2, Stat1 in control and 2OA-BSA groups. j Scatter plot showing the differentially expressed genes in Th1-like cells from the 2OA-BSA group relative to the control group. Red dots represented upregulated genes. Blue dots represented downregulated genes. The P values were estimated in R package seurat using MAST with Bonferroni test adjusted. k Scatter plot showing the top cell type specific regulons in Th1-like cells. l UMAP showing binarized AUC score of Tcf7(+), Klf2(+), Nfatc1(+), Mafg(+). m UMAP showing binarized AUC score of Prdm1(+) Stat1(+) in control and 2OA-BSA groups. n Flow cytometry analysis (top graphs) and percentages (bottom graphs) of CD69+CXCR6+ cells (n = 3 Ctrl, n = 4 2OA), CD69+CD103+ cells (n = 5 Ctrl, n = 5 2OA), CD69+Tbet+ cells (n = 3 Ctrl, n = 4 2OA), CD69+IFN-γ+ cells (n = 3 Ctrl, n = 4 2OA) and CD69-IFN-γ+ cells (n = 3 Ctrl, n = 4 2OA) among CD4+ T cells from the liver samples of 2OA-BSA and Ctrl group mice. Each dot represents a biological replicate from different mice. Box plots show median (center line), the upper and lower quantiles (box), and the range of the data (whiskers). Data are presented as means ± s.d. Significance was determined by two-tailed Student’s t-test, **P < 0.01, ****P < 0.0001. Source data are provided as a Source Data file including exact P values.

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