Fig. 2: HAVDI ligation reduces YAP nuclear translocation independently of viscosity.
From: N-cadherin crosstalk with integrin weakens the molecular clutch in response to surface viscosity
a Schematic representation of YAP translocation to the nucleus of hMSCs on surfaces with RGD and RGD with low and high HAVDI. Image made with BioRender. b Quantification of YAP translocation of hMSCs seeded on SLBs and glass functionalized with RGD and RGD + low and high HAVDI (n from left to right = 36, 36, 32, 15, 34, 21, 20, 21, 16). All n represent cells. Statistical significance was determined using D’Agostino Pearson normality test, followed by a mixed-effects analysis. Quantification of YAP translocation to the nucleus of hMSCs seeded on (c) DOPC (from left to right n = 75, 59, 37, 38), (d) DPPC (from left to right n = 61, 48, 34, 38), and (e) glass (from left to right n = 61, 58, 45, 29) functionalized with low and high HAVDI or scrambled HAVDI peptide; statistical differences in (c–e) are shown only between HAVDI and scrambled HAVDI conditions. Statistical significance was determined using D’Agostino Pearson normality test, followed by a Kruskal−Wallis multiple-comparison test. f Representative images of hMSCs seeded on DPPC functionalized with RGD, RGD + low and high HAVDI or RGD + low and high scrambled HAVDI (scale bar = 50 μm). g–i show early differentiation marker expression measured by In-Cell Western of hMSCs for osteogenesis (7 days) (from left to right n = 9, 5, 7, 9, 8, 7, 9, 9, 7), chondrogenesis (from left to right n = 9, 9, 9, 9, 9, 9, 7, 6, 9) and adipogenesis (5 days) (from left to right n = 6, 5, 3, 8, 6, 7, 6, 9, 9) respectively. In graphs (g–i) n represent areas in the in-cell well. All statistical differences for (g–i) can be seen in Supplementary Tables 2–4. Statistical significance was determined using D’Agostino Pearson normality test, followed by followed by a mixed effects analysis. All data are presented as mean values ± SD. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.
