Fig. 1: RelB deficiency in CD11c⁺ cells results in a reduction of resident cDC2s in gut-draining mesenteric lymph nodes and elevated GATA3⁺ T and Treg cells.

a–f Flow cytometric analysis of T cell populations in mesenteric lymph nodes (mLN) and the lamina propria of the small intestine (SI-LP) of control and RelB^ΔDC mice at steady-state. a, b Representative flow cytometry plots (left) and quantification (right) of frequency of Foxp3⁺ Treg cells among CD4⁺ T cells in mLN (a control n = 9, RelB^ΔDC n = 9) and SI-LP (b control n = 8, RelB^ΔDC n = 8). c, d Representative flow cytometry plots (left) and quantification (right) of frequency of GATA3- and RORγt-expressing Foxp3⁺ Treg cells in mLN (c control n = 9, RelB^ΔDC n = 9) and SI-LP (d control n = 7, RelB^ΔDC n = 8). e, f Representative contour plots (left) and quantification (right) of frequency of GATA3 and RORγt expression in Foxp3⁻ CD4⁺ Th cells in mLN (e, control n = 9, RelB^ΔDC n = 9) and SI-LP (f control n = 8, RelB^ΔDC n = 8). g–i Flow cytometric analysis of dendritic cell populations in mLN of control and RelB^ΔDC mice at steady-state. g Representative contour plots (left) of resident cDCs (rDCs; live dead⁻CD45⁺B220⁻CD64⁻CD11c^highMHCII^int) and migratory cDCs (mDCs; live dead⁻CD45⁺B220⁻CD64⁻CD11c^intMHCII^high) (left) and quantification of indicated frequencies (right) in mLN at steady-state (control n = 9, RelB^ΔDC n = 9). h Representative contour plots (left) of resident DCs subsets (rDC1: XCR1⁺Sirpα⁻; rDC2: XCR1⁺Sirpα⁺) and frequencies (right) in mLN (control n = 9, RelB^ΔDC n = 9). i Representative contour plots (left) of migratory DC subsets (mDC1: CD103⁺CD11b⁻ double-positive DCs (DPDC2): CD103⁺CD11b⁺; single-positive DCs (SPDC2): CD103⁻CD11b⁺) and frequencies (right) in mLN (control n = 9, RelB^ΔDC n = 9). Each dot represents an individual mouse and mean ± SD from two independent experiments is shown. Statistical analysis was performed using two-tailed students t-test. P value of <0.05 was considered statistically significant with *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file.