Fig. 2: RelB is crucial for the differentiation of CIA-DCs in the lamina propria.

a UMAP projection of scRNAseq gene expression profiling from CD11c⁺MHCII^high DCs (among live/dead⁻CD45⁺B220⁻CD64⁻) sorted from the lamina propria of the small intestine (SI-LP) of steady-state control (left) and RelB^ΔDC (right) mice. b Comparison of CIA-DC signature genes across clusters identified in (a). c Representative flow cytometry plots of Plet1⁺CD103⁻ DCs (cryptopatch and isolated lymphoid follicle-associated DCs; CIA-DCs) in SI-LP of control and RelB^ΔDC mice at steady-state (left), quantification of frequencies (middle) and total cell numbers (right) (control n = 5, RelB^ΔDC n = 5). d–i Flow cytometric analysis of T cell populations in mesenteric lymph node (mLN) and SI-LP of control and LtbR^ΔDC mice at steady-state. d, e Representative flow cytometry plots (left) and quantification (right) of Foxp3⁺ Treg cell frequencies among CD4⁺ T cells in mLN (d, control n = 6, LtbR^ΔDC n = 7) and SI-LP (e control n = 3, LtbR^ΔDC n = 4). f, g Representative flow cytometry plots (left) and quantification of frequencies (right) of GATA3- and RORγt-expressing Foxp3⁺ Treg cells in mLN (f control n = 6, LtbR^ΔDC n = 7) and SI-LP (g control n = 3, LtbR^ΔDC n = 4). h, i Representative contour plots (left) and quantification of frequencies (right) for GATA3 and RORγt expression among Foxp3⁻CD4⁺ Th cells in mLN (h control n = 6, LtbR^ΔDC n = 7) and SI-LP (i control n = 3, LtbR^ΔDC n = 4). scRNAseq experiments were performed from sort-purified DCs pooled from three individual mice per genotype. Each dot represents an individual mouse and mean ± SD of two independent experiments is shown. Statistical analysis was performed using two-tailed students t-test. P value of <0.05 was considered statistically significant with *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s. not significant. Source data are provided as a Source Data file.