Fig. 3: RelB regulates gene expression of migratory DCs in the mesenteric lymph node.

a UMAP projection of scRNAseq gene expression profiling from CD11c⁺MHCII^hi DCs (among live/dead⁻CD45⁺B220⁻CD64⁻) sorted from mesenteric lymph node (mLN) of naïve control (left) and RelB^ΔDC (right) mice. b Volcano plot of differentially expressed genes comparing Arc⁻Zeb2⁺ migratory DCs from cluster 2 (control) and cluster 1 (RelB^ΔDC). c Volcano plot of differentially expressed genes comparing Arc⁺Zeb2⁻ migratory DCs from cluster 7 (control) and cluster 8 (RelB^ΔDC). d Heatmap depicting a RelB-dependent core signature in CCR7⁺ DCs in mLN. e Levels of Ccl22, Ccl17, and IL-6 in supernatant of CD11c-enriched cells cultured for 16 h in the absence of any stimulation (control n = 6, RelB^ΔDC n = 5). f, g Representative cluster formation (phase contrast) and fluorescence images (Treg cells: red; non-Treg cells: green) (left) and quantification (right) 270 min (f control n = 4, RelB^ΔDC n = 4) and 360 min (g control n = 4, RelB^ΔDC n = 4) after coculture. h Expression of CD200 (left, control n = 5, RelB^ΔDC n = 4) and CD63 (right, control n = 6, RelB^ΔDC n = 4) in resident and migratory DCs defined in Fig. 1 from mLN of control and RelB^ΔDC mice. i, j Representative contour plots (left) and quantification (right) showing the expression of CD117 in total mLN DCs (i) and resident and migratory DCs and their respective subsets j from control and RelB^ΔDC mice at steady-state (control n = 8, RelB^ΔDC n = 5). scRNAseq experiments were performed from sort-purified DCs pooled from three individual mice per genotype. Each dot represents an individual mouse and mean ± SD of one representative experiment (a–h) or two independent experiments (i, j) is shown. Statistical analysis was performed using two-tailed students t-test. P value of <0.05 was considered statistically significant with *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file.