Fig. 4: RelB^ΔDC mice do not show stronger anaphylaxis in a model of food allergy.

a Experimental scheme of a food allergy regime with OVA as a model antigen for sensitization. CT cholera toxin, OVA chicken ovalbumin. b Core body temperature drop (left) and maximal temperature drop (right) after challenge with OVA on day 35 in control and RelB^ΔDC mice (control (PBS) n = 6, RelB^ΔDC (PBS) n = 6, control (OVA) n = 27 and RelB^ΔDC (OVA) n = 21/25). c OVA-specific IgE levels in serum in food allergic control and RelB^ΔDC mice on day 36 (control before n = 34, RelB^ΔDC before n = 33, control (OVA) n = 24 and RelB^ΔDC (OVA) n = 22). d, e Quantification of intracellular GATA3 and RORγt expression in Foxp3⁻ CD4⁺ Th cells of food allergic mice in mesenteric lymph node (mLN) (d control (PBS) n = 5, RelB^ΔDC (PBS) n = 5, control (OVA) n = 25 and RelB^ΔDC (OVA) n = 22) and lamina propria of the small intestine (SI-LP) (e control (PBS) n = 4, RelB^ΔDC (PBS) n = 5, control (OVA) n = 19 and RelB^ΔDC (OVA) n = 22). f, g Quantification of Foxp3⁺ CD4⁺ T cells (f control (PBS) n = 5, RelB^ΔDC (PBS) n = 5, control (OVA) n = 20 and RelB^ΔDC (OVA) n = 17) and intracellular GATA3 and RORγt expression in Foxp3⁺ CD4⁺ Tregs (g control (PBS) n = 5, RelB^ΔDC (PBS) n = 5, control (OVA) n = 25/20 and RelB^ΔDC (OVA) n = 22/17) in mLN of food allergic animals. h, i Quantification of Foxp3⁺ CD4⁺ T cells (h control (PBS) n = 4, RelB^ΔDC (PBS) n = 5, control (OVA) n = 14 and RelB^ΔDC (OVA) n = 17) and intracellular GATA3 and RORγt expression in Foxp3⁺ CD4⁺ Tregs (i control (PBS) n = 4, RelB^ΔDC (PBS) n = 5, control (OVA) n = 19 and RelB^ΔDC (OVA) n = 22) in SI-LP of food allergic animals. Each dot represents an individual mouse and mean ± SD from two to four independent experiments is shown. Statistical analysis was performed using two-tailed students t-test. P value of <0.05 was considered statistically significant with *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file.