Fig. 5: RelB^ΔDC mice show impaired Th2 effector cell differentiation and parasite clearance after Hpb infection.

a Experimental scheme of primary Heligmosomoides polygyrus bakeri (Hpb) infection and Hpb re-infection (secondary infection) regimen. b, c Intestinal worm counts after primary (day 21, panel b, control (primary Hpb infection) n = 10 and RelB^ΔDC (primary Hpb infection) n = 13) and secondary (day 63, panel c, control (secondary Hpb infection) n = 12 and RelB^ΔDC (secondary Hpb infection) n = 13) Hpb infection of control and RelB^ΔDC mice. d Representative H&E staining of small intestinal tissue sections after secondary Hpb infection. Arrows indicate detected granulomas. e Representative flow cytometry plots (left) and quantification (right) of intracellular GATA3 and RORγt expression among Foxp3⁻ CD4⁺ Th cells from mesenteric lymph node (mLN) of control and RelB^ΔDC mice at steady-state, primary and secondary Hpb infection. Control (steady-state) n = 9, control (primary Hpb infection) n = 10, and control (secondary Hpb infection) n = 12. RelB^ΔDC (steady-state) n = 9, RelB^ΔDC (primary Hpb infection) n = 13, and RelB^ΔDC (secondary Hpb infection) n = 13. f Representative flow cytometry plots of intracellular IL-4 and IL-10 expression (left) and quantification (right) of cytokine expression of Foxp3⁻ Treg cells in mLN after secondary Hpb infection control (secondary Hpb infection) n = 7 and RelB^ΔDC (secondary Hpb infection) n = 7). g Quantification of percentage of resident (rDCs) and migratory (mDCs) cDCs (upper panel), resident DC1s and resident DC2s (lower left panel) as well as migratory DC1s, double-positive DCs (DPDC2) and single-positive DCs (SPDC2) (lower right panel) in mLN at steady-state, after primary and secondary Hpb infection. Control (steady-state) n = 9, control (primary Hpb infection) n = 11, and control (secondary Hpb infection) n = 12. RelB^ΔDC (steady-state) n = 9, RelB^ΔDC (primary Hpb infection n = 13, and RelB^ΔDC (secondary Hpb infection) n = 13. Each dot represents an individual mouse and mean ± SD from at least two independent experiments is shown. Statistical analysis was performed using two-tailed students t-test (b, c, f) or one-way ANOVA with Tukey correction for multiple comparison (e, g). P value of <0.05 was considered statistically significant with *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file.