Table 1 Globally optimized transition rates for 9bp-1A
From: A sequential binding mechanism for 5′ splice site recognition and modulation for the human U1 snRNP
Forward rate | Mean ± SEM (M−1 s−1) | Reverse rate | Mean ± SEM (s−1) | KD ± SE (M) |
|---|---|---|---|---|
\({k}_{{on}}^{1}\) | 1.56 ± 0.01 × 106 | \({k}_{{off}}^{1}\) | 1.53 ± 0.01 × 10−2 | 9.81 ± 0.09 × 10−9 |
\({k}_{{on}}^{2}\) | 2.43 ± 0.30 × 106 | \({k}_{{off}}^{2}\) | 7.69 ± 0.91 × 10−3 | 3.16 ± 0.54 × 10−9 |
\({k}_{{on}}^{3}\) | 4.29 ± 0.06 × 106 | \({k}_{{off}}^{3}\) | 2.59 ± 0.03 × 10−3 | 6.04 ± 0.11 × 10−10 |
\({k}_{{on}}^{4}\) | 1.27 ± 0.03 × 104 | \({k}_{{off}}^{4}\) | 8.40 ± 0.24 × 10−3 | 6.61 ± 0.25 × 10−7 |
