Table 1 Kinetic parameters of SSADHs

From: Adaptive laboratory evolution recruits the promiscuity of succinate semialdehyde dehydrogenase to repair different metabolic deficiencies

Substrate

Protein

App. Km (μM)

kcat (s−1)

kcat/Km (s−1 M−1)

App. Ki (mM)

Succinate semialdehyde

Sad WT

11 ± 1

85 ± 3

7.4 × 106

0.3 ± 0.1

Sad Q262R

5 ± 1

87 ± 4

1.9 × 107

0.5 ± 0.1

GabD

13 ± 1

132 ± 2

1.0 × 107

NA

Erythrose 4-phosphate

Sad WT

2,102 ± 328

1.1 ± 0.1

5.3 × 102

NA

Sad Q262R

28 ± 4

3.0 ± 0.1

1.1 × 105

7.4 ± 3.3

Glyceraldehyde 3-phosphate

Sad WT

1,106 ± 92

3.1 ± 0.2

2.8 × 103

NA

Sad Q262R

67 ± 9

19 ± 1

2.8 × 105

1.4 ± 0.3

  1. Measurements were carried out in 75 mM HEPES pH 8.0, 37 °C in the presence of 1 mM NAD+, or NADP+ in the case of GabD. The data present means ± SD, N = 3, as computed from nonlinear regression (Supplementary Fig. 5). Source data are provided as a Source Data file.
  2. App. apparent, NA not applicable.
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