Fig. 2: Conformational dynamics of tβ1AR upon binding to agonist. | Nature Communications

Fig. 2: Conformational dynamics of tβ1AR upon binding to agonist.

From: Ligand-induced conformational changes in the β1-adrenergic receptor revealed by hydrogen-deuterium exchange mass spectrometry

Fig. 2

A Chemical structure of agonist isoprenaline, comparative HDX-MS results for tβ1AR + isoprenaline vs tβ1AR, deuterium uptake plots for ICL1 and TM5. B Chemical structure of endogenous agonist norepinephrine, ΔHDX-MS results for tβ1AR + norepinephrine vs tβ1AR, deuterium uptake plots for ICL1 and TM5. Results are mapped on tβ1AR structure (modelled using PDB structures; 2VT4 chain A and 6IBL chain A). Ser residues, crucial for binding the ligands into catecholamine binding pocket of the receptor, are highlighted in red. Relative deuterium uptake shows cumulative data across all time points for every receptor-ligand complex. Data are presented as mean values ± SD, where error bars represent the standard deviation at each time point. Each measurement is based on three technical replicates (n = 3). Source data are provided as a Source Data file.

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