Fig. 4: ΔHDX analysis of tβ1AR apo vs tβ1AR bound to isoprenaline, colterol HCl and orciprenaline. | Nature Communications

Fig. 4: ΔHDX analysis of tβ1AR apo vs tβ1AR bound to isoprenaline, colterol HCl and orciprenaline.

From: Ligand-induced conformational changes in the β1-adrenergic receptor revealed by hydrogen-deuterium exchange mass spectrometry

Fig. 4

A Chemical structure of tested ligands, full agonist isoprenaline and its derivatives colterol hydrochloride and orciprenaline. B Regions showing significant difference in HDX for tβ1AR with isoprenaline derivatives mapped onto β1AR structure (modelled using PDB structures; 2VT4 chain A and 6IBL chain A). Relative deuterium uptake shows cumulative data across all time points for every receptor-ligand complex. C Uptake plot for ICL1 displaying differences in relative deuterium uptake (Da) between tβ1AR Unbound (apo) and tβ1AR + isoprenaline, tβ1AR+colterol HCl, and tβ1AR + orciprenaline, respectively. Data are presented as mean values ± SD, where error bars represent the standard deviation at each time point. Each measurement is based on three technical replicates (n = 3). Source data are provided as a Source Data file.

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