Fig. 2: Carbohydrate metabolism promotes pyrazine biosynthesis in K. oxytoca.
From: Mucosal sugars delineate pyrazine vs pyrazinone autoinducer signaling in Klebsiella oxytoca
a Representative HPLC-QTOF-MS total ion chromatograms (n = 3 biological replicates) of wildtype K. oxytoca, ∆nanA K. oxytoca, and medium control samples supplemented with Neu5Ac. Pyrazine compounds are numbered in black; yersiniabactin compounds are numbered in gray. b Molecular networking analysis of the pyrazine molecular family. Connected nodes share high degrees of similarity in their MS2 fragmentation patterns. c Molecular structures of K. oxytoca pyrazines. Key NMR signals are indicated for isolated pyrazines. Pyrazine scaffolds are highlighted in green. d Relative abundances of identified pyrazines between wildtype K. oxytoca and ∆nanA K. oxytoca supplemented with Neu5Ac (n = 3 biological replicates). Pyrazines were not detected in the growth medium control samples. P-values for the production of all compounds between wildtype and ∆nanA groups are < 0.0001. e Relative production of pyrazines 5–9 and yersiniabactins 10 and 11 in wildtype and ∆nanA K. oxytoca strains (n = 2 biological replicates) when supplemented with D-Galactose or Neu5Ac and D-Galactose, respectively. Representative 10b was chosen for integration. Statistical analyses performed through an unpaired two-tailed t-test; n.s. indicates a non-significant difference, * indicates p < 0.05; ** indicates p < 0.01, and *** indicates p < 0.001. Data are represented as mean values +/– SD.
