Fig. 6: Distribution of pyrazine biosynthetic machinery across bacteria.
From: Mucosal sugars delineate pyrazine vs pyrazinone autoinducer signaling in Klebsiella oxytoca
a Phylogenetic analysis of Pyr homolog-containing operons across various families of bacteria. Bacterial families are colored as follows: green, Enterobacteriaceae; red, Pseudomonoadaceae; blue, Francisellaceae; olive, Streptomycetaceae; and black, other. Functional annotations of the genes are separately color coded and displayed in the top-right insert. b Protein similarity networking analysis of the Pyr protein across various families of bacteria. Connected nodes indicate high protein similarity (>90% sequence homology). Bacterial families are color coded and displayed in the bottom-right insert. The Pyr network that contains Klebsiella species (in the Enterobacteriaceae family) is boxed. c Production of pyrazines 5–9 and monomers 12 and 13 by select Pyr homologs in the heterologous host E. coli BL21(DE3). The left axis plots K. oxytoca, K. pneumoniae, and E. coli (STEC) integrated areas, while the right axis plots the F. tularensis integrated areas. Data are represented as mean values +/– SD.
