Fig. 1: Copy number and tandem repeat patterns of ddp-BAC.
From: Evolutionary engineering of methylotrophic E. coli enables fast growth on methanol
a A schematic showing BAC with and without the ddp operon. b The copy number of gfp measured by droplet digital PCR (ddPCR). Each strain was tested with at least 3 individual colonies from a LB plate. Only ddp-BAC with the ddp operon in the recA+ host existed in high copies (BW25113 strain with ddp-BAC::gfp, n = 6; BW25113 strain with BAC::gfp and ΔrecA strain with BAC::gfp or ddp-BAC::gfp, n = 3; all the data are biological repeats; data are presented as mean values ± SD). c Nanopore sequencing of ddp-BAC. The patterns shown are raw intact nanopore reads mapped to BAC. The orange label represents the ddp operon, while the blue arrows represent the BAC backbone with gfp. Only ddp-BAC in the recA+ host showed tandem repeats with a distribution of copy numbers. Source data are provided as a Source Data file.
