Fig. 3: Debottlenecking evolution using ddp-BAC::RHTTP.
From: Evolutionary engineering of methylotrophic E. coli enables fast growth on methanol
a Formaldehyde concentration in SM1 cultures at an OD600 of around 1.0, grown in cell culture tubes with low (400 mM, M400) and high (1000 mM, M1000) methanol concentrations. (n = 5 for each group, biological repeats; data are presented as mean values ± SD). b Doubling time of the SM1 strain evolved up to passages 77 showed minor improvement. c SM1p with various plasmids harboring rpe, hps, tkt, tal, phi (RHTTP) grown and passed in 1000 mM methanol. Each strain was tested with at least 3 colonies from a LB plate. d The growth curves and the doubling time of SM7 grown in 400 mM methanol. The different colors represent different biological repeats (n = 3). Source data are provided as a Source Data file.
