Fig. 4: The evolution process towards SM8.
From: Evolutionary engineering of methylotrophic E. coli enables fast growth on methanol
a The copy number of ddp-BAC and growth rate changes along the evolution line towards SM8. The copy number of ddp-BAC was determined from Illumina sequencing. The purple line corresponds to the copy number of ddp-BAC. The red line indicates the growth rate in 400 mM methanol, while the gray line represents the growth rate in high methanol (1000 mM or 1200 mM). The red dashed line represents the growth rate where mutS is not restored. (Each passage is defined as a subculturing step, growing from OD600 0.05–0.1 to OD600 1). kb refers to kilobases, where it depicts the unit size of IS5-flanked tandem repeats derived from the original 70 kb tandem repeats. b The overall accumulation of different kinds of mutations throughout the evolution line, including SNP, INDEL, and Silent mutations. The numbers depict the passage number of the strain. All dots represent samples that were sequenced by NGS. Source data are provided as a Source Data file.
