Fig. 6: Characterization of DPC in SM7.
From: Evolutionary engineering of methylotrophic E. coli enables fast growth on methanol
a Time courses of SM1 and SM7 grown in 400 mM MeOH. Samples were collected at time points I to VI for SM1, and a to f for SM7 respectively, for extracellular formaldehyde measurement using LC-MS/MS. b Transmission electron microscopy (TEM) images of DPC products extracted from both SM1 and SM7 at different growth stages by negative staining. The experiment was repeated twice independently with similar results. c Enzymatic assays of Mdh and enzymes in the RuMP cycle in SM1 and SM7. Mdh activity was measured by the colorimetric Nash assay. The remaining enzymes were measured using coupled enzyme assays with a NADPH readout at 340 nm (n = 3, biological repeats; data are presented as mean values ± SD). Source data are provided as a Source Data file.
