Fig. 6: Tissue-specific expression of detoxifying enzymes (glutathione S-transferases), efflux transporters (ABC transporters) and Ly6 proteins. | Nature Communications

Fig. 6: Tissue-specific expression of detoxifying enzymes (glutathione S-transferases), efflux transporters (ABC transporters) and Ly6 proteins.

From: Spatial transcriptomics of a parasitic flatworm provides a molecular map of drug targets and drug resistance genes

Fig. 6

a Spatial projections showing expression patterns of F. hepatica glutathione S-transferases (GSTs). Mu-class GSTs: Mu28/7 (D915_008526), Mu26/51 (D915_008966), Mu26/51-like (D915_008366), Mu29/1 (D915_007977), Mu27/47 (D915_010266), Mu5 (D915_002901); Sigma-class GSTs: S1 (D915_001201), S2 (D915_006844); Omega-class GSTs: O1 (D915_001421), O2 (D915_001777); and microsomal GSTs: m1 (D915_007840), m2 (D915_002950). Zeta class GST (D915_006391) is shown in Supplementary Fig.Ā 9a. For GST phylogeny, see Supplementary Fig.Ā 10a. b Spatial projection showing expression patterns of selected F. hepatica ABC-B transporters. See Supplementary Fig.Ā 9b for spatial projections of ABC-B genes not shown in (b). For ABC-transporter phylogeny, see Supplementary Fig.Ā 10b. c Left: Heatmap of GSTs, ABC-B transporters and Ly6 proteins showing the average expression per cluster (mean of UMI counts, normalized and scaled). Please note: While spatial plots (a, b, d) are shown for only one representative section, the heatmap includes expression data from all four tissue sections in the dataset. Right: Heatmap showing the average spot expression of those genes in the whole dataset (log1p normalized counts). Source data are provided as a Source Data file. The two testis-associated ABC-B transporters (D915_008893 and D915_004337) and D915_007290, which is preferentially expressed in the ovary, are not shown in (b). See Supplementary Fig.Ā 9b for their spatial expression patterns. d Spatial projections showing expression patterns of tegumental, parenchymal and potentially intestinal F. hepatica Ly6 proteins. Tegumental Ly6 proteins: Ly6-B (D915_008996), Ly6-F (D915_008863), Ly6-N (D915_007373), Ly6-Q (D915_008394). Ly6 proteins with prominent expression in the parenchyma: Ly6-D (D915_006706), Ly6-A (D915_001097), Ly6-U (D915_000333). Ly6 proteins expressed in the gut cluster: Ly6-K (D915_009743), Ly6-L (D915_008235). See Supplementary Fig.Ā 9c for Ly6 proteins not shown in (cā€“d). a, b, d Expression level encoded by color (gray = low, red = high).

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