Fig. 1: Thermal proteome profiling (TPP) of FBP-interacting proteins.
a TPP workflow for quantitative chemical proteomic profiling of FBP-interacting proteins in HepG2 cell lysates. b Venn diagram for the datasets of proteins identified by TPP strategy from three biological replicates. c Volcano plot of the identified proteins by TPP strategy from HepG2 cell lysates by FBP treatment and control samples. Dashed lines indicate p =  0.05, 1.5-fold change in three replicates from two-sided t-test. Key hits are highlighted for proteins with increased stability (red), proteins with reduced stability (blue) and proteins without stability change (gray). d Molecular function and subcellular distribution analysis of enriched 66 FBP targets. Statistical differences were determined by a one-sided Fisher’s Exact test. e Functional pathways analysis of enriched 66 FBP targets. Statistical differences were determined by a one-sided Fisher’s Exact test. f MS1 chromatographic peaks of the representative peptides from each protein with calculated 57 °C versus 37 °C ratios for cell lysates treated with either FBP or H2O (Blue traces represent the chromatographic trace of FBP target at 37 °C; red traces represent the chromatographic trace of FBP target at 57 °C; green lines designate the integration range for each peak quantification).
