Fig. 2: Validation of the identified FBP targets. | Nature Communications

Fig. 2: Validation of the identified FBP targets.

From: Thermal proteome profiling reveals fructose-1,6-bisphosphate as a phosphate donor to activate phosphoglycerate mutase 1

Fig. 2

a CESTA workflow for validation of FBP-interacting proteins. HepG2 cell lysates were treated with FBP or H2O, then subjected to immunoblotting. b Thermal shift curves of FBP targets with increased thermal stability by immunoblotting in HepG2 cell lysates, including PGAM1, PGM1, PMM2 and PRPS1. Blue curves represent FBP treatment and black curves represent H2O treatment (control). c Thermal shift curves for FBP targets with reduced thermal stability, including GARS, HDAC2, POLR2A and PDXDC1. d Thermal stability changes of recombinant proteins incubated with FBP or H2O by Coomassie brilliant blue (CBB) staining (n = 3 independent experiments with similar results). All measurements are presented as mean ± SD for three biological replicates. Source data are provided as a Source Data file.

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