Fig. 2: GAD-specific cells show qualitative alterations in T1D patients.
From: Physiological and pathogenic T cell autoreactivity converge in type 1 diabetes
a UMAP plot for all GAD-specific cells coloured per cluster. b Details of gene expression per cluster, showing the fraction of cells expressing a gene (dot size) and their expression level (colour). c UMAP plot coloured per type of donor. d CTLA-4 expression levels in cluster #4 (two-sided Mann Whitney U test with Bonferroni multiple comparisons correction. p = 0.043). e IKZF2 expression levels in cluster #10 (two-sided unpaired t test with Welch’s correction. p = 0.0034). f CXCR5 expression levels in cluster #8 (two-sided Mann Whitney U test with Bonferroni multiple comparisons correction. p = 1.97 × 10−5). g Distribution of HD and T1D cells within cluster #7 subclusters (two-sided Chi-Square test. p = 0.047). h Frequencies of transcription factor-based cell subsets in FOXP3+ and FOXP3neg cells of cluster #5. i CCR5 and Egr2 expression levels in cluster #2 (two-sided Whitney U test with Bonferroni correction. p = 2.7 × 10−3 and p = 1.4×10-6 respectively). j Cell surface phenotype distributions (two-sided Chi-square test. p < 0.0001). c, d, e, f, i blue, HD; red, T1D. g, j white, HD; black, T1D. *: p < 0.05. **: p < 0.01. ***: p < 0.001. ****: p < 0.0001.
