Fig. 5: GAD-specific TCRB CDR3 nucleotide sequences are public and frequent in specific peripheral immune cell subsets.
From: Physiological and pathogenic T cell autoreactivity converge in type 1 diabetes
a, b Percentage of individuals where GAD- (a) and CMV- (b) specific TCRB CDR3 nucleotide sequences are tracked back. Although each line represents a clonotype, note that many clonotypes show the same behaviour and, as such, they are overlapped in this representation. c Intraindividual frequencies of GAD- and CMV- specific TCRB CDR3 nucleotide sequences (GAD from HD: 1 in TN, 19 in CM, 6 in Treg. GAD from T1D patients: none in TN, 26 in CM, 5 in Treg. CMV: 3 in TN, 32 in CM, 3 in Treg, 6 in Tscm) found in the peripheral immune repertoires (Kruskal Wallis+Dunn). One CMV clonotype exceptionally frequent in the CM subset is not depicted in the figure to ease the visualisation of all other clonotypes (frequency=0.002529). d Ratios of the frequencies of GAD-specific TCRB CDR3 nucleotide sequences in Treg and CM subsets from the same donor (two-sided Mann-Whitney U test). c, d Blue squares: GAD-specific TCRB CDR3 nucleotide sequences found in HD. Red triangles: GAD-specific TCRB CDR3 nucleotide sequences found in T1D patients. White circles: CMV-specific TCRB CDR3 nucleotide sequences. Error bars represent standard deviations. *: p < 0.05.
