Fig. 4: NANOG upregulates ECM organization and Nerve Regeneration pathways and increases myofiber cross section area 5 weeks after nerve injury.

A Top 10 most highly enriched GO cellular components, molecular functions and biological processes 5 weeks after PNI. ECM: Extracellular Matrix, RTK: Receptor Tyrosine Kinase. B Enrichment plots from Reactome database depicting ECM synthesis and signaling associated pathways; ECM organization, Collagen formation, Integrin cell surface interactions and ECM proteoglycans. C Enrichment plots from KEGG and Reactome databases depicting nerve development pathways; Axon guidance, Gap junction, Neuroactive ligand receptor interaction and NCAM signaling for neurite outgrowth; n = 6 biological replicates for both WT and NANOG. D Immunostaining of TA muscle sections for Laminin (red) and DAPI (Blue) 5 weeks after nerve injury. Scale bar = 100 µm. Areas within white circles depict atrophied muscle fibers. E Quantification for myofiber cross sectional area in WT transected, NANOG Transected and Control Non-Transected muscle; n = 6 biological replicates for all groups- Control non-transected, WT transected and NANOG transected, 200-250 muscle fibers per mouse. Data is presented as Mean ± SEM. Statistical analysis performed using two-way ANOVA with Tukey’s multiple comparison test. P-values depicted in the plot represent statistically significant differences in WT transected and NANOG transected groups. Source data are provided as a Source Data file.