Fig. 3: THBS1 is epigenetically primed in late irradiated dermal fibroblasts.

A qRT-PCR of THBS1 in paired RT⁻ and RT⁺ fibroblasts (n = 7 donors). B Integrative genomics viewer image of THBS1 genomic loci with ATAC signals in paired RT⁻ vs. RT⁺ fibroblasts (n = 5 donors). The analysis of TOBIAS footprinting in the paired RT⁻ and RT⁺ fibroblasts is shown in gray and pink tracks. The green bar indicates a predicted promoter region of THBS1 and two RUNX1 binding motifs are identified. C Paired RT⁻ and RT⁺ fibroblasts were treated with or not with TGF-β for 24 hours and the cells were harvested for ChIP-qPCR detecting RUNX1 binding (D) and H3K4me1 histone modification (E) at the THBS1 gene (n = 2), and qRT-PCR of THBS1 expression (n = 3) (F). G Fibroblasts from RT⁻ skin or skin of healthy donors were irradiated and harvested 6 hours − 2 weeks post-irradiation. qRT-PCR analysis of CDKN1A H), PCNA I, and THBS1 (J) in non-irradiated vs. irradiated fibroblasts (n = 3). Fibroblasts were irradiated and then treated with or not with TGF-β 1 day (n = 3) K, or 6 days post-irradiation (n = 4) L. THBS1 expression was analyzed by qRT-PCR. Data are presented as means ± SD D, F, H–J, K, L. Paired two-tailed student’s t-test (A) or two-way ANOVA F, H–J, K, L. Source data are provided as a Source Data file.