Fig. 2: Mini-tissue-like properties of TB.
a, b The scaffold unit morphology and pores are labeled in red (b1–b4 represents the single pore in one square). c TB wrapped ganglia. d Pore size analysis. e, f Comparison of stress-strain curves and tensile modulus between strength and non-strength scaffold. n = 3. g Confluency ratio on TB with 100k, 300k, and 500k cell seeding at 1.5 h, 1 day, 3 days, and 5 days. n = 9. Data are presented as mean ± SEM. h Cell loading capacity between petri dish (2D) and TB. n = 7. i The EdU images of 2D and TB. j, k Flow cytometry of cells on 2D or TB with EdU stain. l Expression ratio in G1, S, and G2 phase in flow cytometry. n = 8. m Proliferation evaluation of 2D and TB by CCK8 kit. n = 3. n TB expressed Vinculin, ZO-1, and CD31. o TJs formation on TB, labeled with a red circle. p A voidless sheet structure of TB. q–s Flow cytometry characterization and expression ratio of TB positively defined as VE, VEGFA, ZO-1, and Vinculin. n = 4. t Live/dead cell comparison among 2D, fresh TB (Fresh), TB thawed from 10% v/v DMSO medium at 3 weeks (DMSO-3w), TB thawed from a serum-free cryopreservation medium (SFCM) at 3 weeks (SFCM-3w), 5 months (SFCM-5m), and 1 year (SFCM-1y). n = 3. For bar graphs, data are presented as mean ± SEM. For box plots, box shows median, 25th and 75th percentiles and whiskers represent minima and maxima. A two-tailed t-test was employed to compare two groups, followed by multiple comparisons with Bonferroni correction. Comparison involving more than two groups entailed one-way ANOVA, followed by a variance homogeneity test with Levene correction and multiple comparisons with Bonferroni correction. Source data are provided in NC._data_file_s1.
