Fig. 6: Histomorphology changes and axon variations in MPG at 2 weeks.

a, b The overview morphology and representative H&E images of MPG. Bleeding is labeled by →, necrosis is indicated by ▲, and neutrophil infiltration is labeled by the yellow dot. c, f Nissl bodies stained by toluidine blue in MPG and analysis of its quantity per slice. n = 5. Vacuolization is indicated by the red dot. d, g IHC images of CD31 and expression in MPG per slice. Arteriole labeled by * and venule labeled by ^#. n = 5. e TEM micrograph of myelinated axons (ax), mitochondria (mit), and myelin sheath (MS) in MPG. h G-Ratio raincloud plot with a box, box shows median, 25th and 75th percentiles and whiskers represent minima and maxima. n = 27 in 4 MPGs. i Correlation between G-Ratio perimeter and G-Ratio area. n = 27 in 4 MPGs. For bar graphs, data are presented as mean ± SEM. For box plots, box shows median, 25th and 75th percentiles and whiskers represent minima and maxima. One-way ANOVA followed by a variance homogeneity test with Levene correction and multiple comparisons with Bonferroni correction. Outliers exceeding upper and lower limits are removed based on box plot analysis. Source data are provided in NC._data_file_s1.