Fig. 1: Preferred orientation of V1 neurons drifts over time.
From: Sensory experience steers representational drift in mouse visual cortex
a Example field of view from one mouse. Top: Average GCaMP6s (green) and mRuby2 (red) fluorescence, scale bar 100 µm. Bottom: imaging timeline of six mice, white squares are days with imaging sessions. b Correlation of the pairwise signal correlation (PSC) matrices compared across all imaging sessions of one mouse. Red: exponential decay fit (\(y=0.31+0.43{e}^{-0.09*x}\)). c Left: responses of an example neuron on 3 days. Single-trial responses, gray, average black. Gray bars: stimulus window of 5 s, grating directions indicated above. Scale bar: 100 ΔF/F. Right: polar plots of the responses on the left, with gray lines indicating mean response. Preferred orientation (PO) in blue with 95% confidence intervals (CI) as blue dashed lines. d Left: POs 1 day apart. Two-sided circular-circular Pearson’s correlation r = 0.968 (p < 1 × 10−16), n = 781 PO changes from 169 neurons from six mice. Right: POs 20 days apart. r = 0.800 (p < 1 × 10−16), n = 360 PO changes from 170 neurons from six mice. Red: significant changes, gray: non-significant changes. e Percentage of concurrently tuned cells that significantly changed their PO vs. interval length. Six individual mice as gray lines, with black line as mean with error bars as S.E.M. One-way ANOVA F(61)14.98, p = 5.62 × 10−14. Asterisks indicate Dunnett’s post hoc test (1–2 days vs. all), p < 0.05. f Cumulative probability distributions of the absolute size of PO changes (|∆PO|; drift magnitude) for different intervals. g Median drift magnitude for all PO changes in black and only significant PO changes in red. Error bars are bootstrapped 95% CIs. Two-sided Kruskal–Wallis test on all PO changes (χ2(12) 864, p = 2.9 × 10−177, n = 468–4194 PO changes), and on only significant PO changes (χ2(12) 122, p = 2.4 × 10−20, n = 188 to 347 PO changes). Asterisks indicate Bonferroni corrected two-sided Mann–Whitney U tests between 1 and 2 days vs. all other intervals p < 0.05. h Cumulative probability distribution of drift magnitude. Cyan: 1–2 day intervals, n = 2147 PO changes from five mice; magenta: 19–20 day intervals, n = 657 PO changes from five mice; black: changes between still trials and running trials within sessions, n = 152 PO changes from five mice. Two-sided Mann–Whitney U test for within-session changes between running and still trials compared to changes across short time intervals (U = 9.78 × 106, p = 1.04 × 10−5), or long time intervals (U = 1.11 × 106, p = 2.13 × 10−8). Source data are provided as a Source Data file.
