Fig. 3: Persistent immature neurophysiological signature of VTA dopamine neurons in mice exposed to NIC in adolescence. | Nature Communications

Fig. 3: Persistent immature neurophysiological signature of VTA dopamine neurons in mice exposed to NIC in adolescence.

From: Transient nicotine exposure in early adolescent male mice freezes their dopamine circuits in an immature state

Fig. 3

A Experimental design for patch clamp experiments. B Left: No difference in peak amplitude of nicotine current between adult mice that received NIC or SAC as adolescents (NIC N = 2 mice, n = 14 neurons; SAC N = 2 mice, n = 13 neurons, Table 2A). Right: No difference in peak amplitude of nicotine current between adult mice that received NIC or SAC as adults (NIC N = 5 mice, n = 11 neurons; SAC N = 5 mice, n = 9 neurons, Table 2B). C Left: AMPA/NMDA ratio was decreased in mice that received NIC as adolescents (NIC N = 4 mice, n = 11 neurons; SAC N = 4 mice, n = 11 neurons, Table 2C). Right: NMDA current decay (weighted τ) was increased in mice that received NIC as adolescents (NIC N = 4 mice, n = 11 neurons; SAC N = 4 mice, n = 11 neurons, Table 2D). D Left: No differences in AMPA/NMDA ratio were observed when mice received NIC as adults (NIC N = 4 mice, n = 8 neurons; SAC N = 5 mice, n = 9 neurons, Table 2E). Right: NMDA current decay (weighted τ) was the same between mice that received NIC or SAC as adults (NIC N = 4 mice, n = 8 neurons; SAC N = 5 mice, n = 9 neurons, Table 2F). All bar graphs are presented as mean values ± SEM. *p < 0.05, **p < 0.01, ***p < 0.01. All statistical comparisons were two-sided. Detailed information about statistical testing is available in Table 2. Source data are provided as a Source Data file.

Back to article page