Fig. 3: Characterization of systemic cells, mucosal dendritic cells, and mucosal NK cells/ innate lymphoid cells (ILCs) in rhesus macaques. | Nature Communications

Fig. 3: Characterization of systemic cells, mucosal dendritic cells, and mucosal NK cells/ innate lymphoid cells (ILCs) in rhesus macaques.

From: Loss of HIV candidate vaccine efficacy in male macaques by mucosal nanoparticle immunization rescued by V2-specific response

Fig. 3

a Correlation between efferocytosis MFI in blood at week 20/pre and number of intrarectal challenges in the V2-TTB NP group (n = 12, p = 0.02). b Comparison of normalized trogocytosis using antibody-free controls as denominator in plasma against ∆V1 gp120 protein among different groups of animals (V2-TTB NP, n = 12; TTB NP, n = 9; empty NP, n = 9; systemic vaccine, n = 14). c, d Correlation of trogocytosis in plasma against ∆V1 gp120 protein c at week 24 with VL at 1 wpi in V2-TTB NP group (n = 10, p = 0.004) and d at week 17 with VL at 8 wpi in the systemic vaccine group (n = 9, p = 0.008). e Correlation of myeloid dendritic cells in rectal mucosa at week 17 with VL at 12 wpi in V2-TTB NP group (n = 10, p = 0.03). f Comparison of plasmacytoid dendritic cells in rectal mucosa at week 17 in all groups (V2-TTB NP, n = 12; TTB NP, n = 9; empty NP, n = 8). g, h Correlation of plasmacytoid dendritic cells in rectal mucosa at week 17 with g intrarectal challenges in TTB NP group (n = 9, p = 0.03) and h VL at 8 wpi in empty NP group (n = 8, p = 0.02). i Comparison of NKp44+ ILCs in rectal mucosa at week 17 in all groups (V2-TTB NP, n = 12; TTB NP, n = 9; empty NP, n = 9). j Correlation of NKp44+ ILCs with number of intrarectal challenges in V2-TTB NP group (n = 12, p = 0.03). k Comparison of PMA/Ionomycin-induced IFN-γ+ NKG2A-NKp44- ILCs in rectal mucosa at week 17 in all groups (V2-TTB NP, n = 12; TTB NP, n = 9; empty NP, n = 9). l–n Correlation of PMA/Ionomycin-induced IFN-γ+ NKG2A-NKp44- ILCs with l VL at 2 wpi in V2-TTB NP group (n = 10, p = 0.02), m VL at 4 wpi in TTB NP group (n = 8, p = 0.03), and n myeloid dendritic cells in rectal mucosa at week 17 in TTB NP group (n = 9, p = 0.04). Data shown in b, f, i, k were analyzed with the two-sided Mann-Whitney test. Data shown in a, c–e, g, h, j, l–n were analyzed with the two-sided Spearman correlation test. Horizontal and vertical bars denote mean and SD. Source data are provided as a Source Data file. Here, the black, red, blue, and purple symbols represent male macaques immunized with systemic vaccine, vaccine combined with V2-TTB NP, vaccine combined with TTB NP, and vaccine combined with empty NP, respectively.

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