Fig. 7: Tumor immune microenvironment of copy number alteration (CNA)-quiet (n = 12) and CNA-other (n = 27) oral cavity SCC (OCSCC).
From: Hallmarks of a genomically distinct subclass of head and neck cancer
a Representative image stained with a seven-color multiplex immunohistochemistry (mIHC) Opal panel characterizing CD44v6+ tumor cells, CD163+ macrophages, CD19+ B cells, CD8+ T cells, CD3+ CD8- FoxP3- T cells (CD4+ T helper cells), and FoxP3+ regulatory T cells (Tregs, CD3 + , CD8-). Immune cell densities were determined within the tumor center (TC), invasive margin (IM), consisting of a 250 µm inner and outer margin, as well as the tumor area (TA), which is the sum of the TC and IM. b Total immune cell (T cells, B cells, and CD163+ macrophages together) densities (in cells/mm2) in tumor area, tumor center, and invasive margin of CNA-quiet (n = 12, yellow) and CNA-other (n = 27, blue) OCSCC. c Densities (in cells/mm2) per immune cell type in the tumor area of CNA-quiet (n = 12) and CNA-other OCSCC (n = 27). d Ratio of the densities CD8+ T cells and CD4+ Th cells to FoxP3+ Tregs in the tumor center of CNA-quiet (n = 12) and CNA-other OCSCC (n = 27). e Representative mIHC image of CNA-quiet (left panel) and CNA-other tumor (right panel) with respectively high and low CD8 + T cell to FoxP3+ T cell ratios. f Percentage of target cells (yellow) within 10 µm radius of a reference cell (blue). g Percentage of CD8+ T cells and CD4+ Th cells within 10 µm radius of a FoxP3+ Treg in the center of CNA-quiet (n = 12) and -other OCSCC (n = 27). h Representative mIHC image of CNA-quiet (left panel) and CNA-other tumor center (right panel) with respectively low and high percentage T cells within 10 µm radius of a FoxP3+ Treg. i Percentage of CD8+ T cells and CD4+ Th cells within 10 µm radius of a tumor cell in the invasive margin of CNA-quiet (n = 12) and CNA-other OCSCC (n = 27). j Representative mIHC image of CNA-quiet (left panel) and CNA-other tumor center (right panel) with respectively high and low percentage T cells within 10 µm radius of a tumor cell. p-values were obtained by multiple unpaired non-parametric two-sided Mann-Whitney tests. Data are presented as mean and error bars represent standard deviation. Source data are provided with this paper.
