Fig. 5: Polar localization of CpdR substrates is correlated with increased rate of degradation. | Nature Communications

Fig. 5: Polar localization of CpdR substrates is correlated with increased rate of degradation.

From: Phospho-signaling couples polar asymmetry and proteolysis within a membraneless microdomain in Caulobacter crescentus

Fig. 5

a Localization of YFP-tagged substrates in ∆cpdR; popZ::mChy-popZ and ∆popZ C. crescentus strain backgrounds. Scale bar = 5 μm. b Time-lapse images of YFP-tagged substrate localization in a WT C. crescentus background, at 4 min intervals. Blue arrows mark frames with foci in stalked cell, orange arrows mark frames with foci in swarmer cell. Pink bar idicates the time of cell separation. After accounting for photobleaching and temporally alignging the cells with respect to the time of cell separation, average fluorescence intensities for stalked and swarmer cell bodies, normalized to maximum fluorescence intensity, were plotted against time (line graphs, with error bars showing standard deviation, n = 20 cells). The fractions of cells ehxhibiting a polar focus, normalized to the highest value observed, were plotted on the same time axis (bar charts). Scale bar = 2 μm. c Degradation of HA-tagged proteolysis substrates following inducer wash-out, observed by western blotting with α-HA antibody. Average band intensities from three separate experiments were plotted against time (graphs, bar = standard deviation). Source data are provided as a Source Data file.

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