Fig. 2: MLL oncoprotein levels scale with target gene expression.

a Genome Browser tracks show the difference in MLL N-terminal (red) and C-terminal (blue) signals in three MLL::AF4 bearing leukemias, over the most common oncoprotein-target locus: MBNL1 (bottom black bar = oncoprotein-target peak). The USP5 promoter is an example of a wild-type MLL target site (bottom black bar = wild-type MLL peak). AF4 (brown) DOT1L (cyan) ENL (magenta) and H3K4me3 (green) signal is also shown for the p454 sample. b Oncoprotein scores over MBNL1 (quantified over the bottom-black bar in (a)) are representative of the average oncoprotein scores in each UMAP cluster; p values were computed using a two-tailed independent samples t-test; Cluster 1 n = 13 samples, Cluster 2 n = 6 samples, Cluster 3 n = 15 samples, Cluster 4 n = 5 samples; boxplot center lines = median, box limits = first and third quartiles, whiskers = 1.5 times the interquartile range (IQR). c The MBNL1 oncoprotein scores are correlated with the relative expression of MBNL1 as measured by qPCR; dotted lines indicate the regression and R² measures the fit to the data, error bars indicate the standard deviation of the mean of three qPCR biological replicates. d ENL is significantly enriched over the oncoprotein-target sites. p values were computed using a two-tailed independent samples t-test; n values are listed as wild-type site #, oncoprotein site #: SEM = 15611, 491; p318 = 22014, 246; p279 = 25672, 272; p454 = 18509, 438; boxplot center lines = median, box limits = first and third quartiles, whiskers = 1.5 times the interquartile range (IQR). Source data are provided as a Source Data file.