Fig. 2: Synthesis and characterization of PCW Nanoflowers.

a Preparation of PCW nanoflowers by self-assembly of PA, Cu2+, and Lyw. Created in BioRender. Ji, J. (2024) BioRender.com/g49x121b TEM images of PC nanopetals and PCW nanoflowers. Inset: The digital photos of PC and PCW in aqueous solution. c Zeta potential values of PC, PCW, PA, and Lyw. d Intensity-average hydrodynamic diameters of PC and PCW. e TEM, HR-TEM, and SAED images of PCW. f TEM mapping analysis of PCW with different colored dots representing C, N, Cu, and S. g Adsorption-desorption isotherm of PCW. h XPS survey spectrum of Cu2p in PCW. i FT-IR spectra of PC, PCW, PA, and Lyw. j UV-vis absorption spectra of PC, PCW, PA, and Lyw. k TEM images of PCW at pH 6.0 and pH 5.0, respectively. l Release curves of Cu2+ from PC and PCW over time in aqueous solution. m Release curves of Cu2+ from PC and PCW over time in MOPS buffer with different pH. b, e, f, k showed one of three independent experiments with similar results. Data for (c) were presented as mean values ± standard deviations (SD) (n = 3 independent experiments). error bars = SD. Source data were provided in Source Data file.