Fig. 1: Illustration of the scCode-fection technique by using hierarchically assembled Knock-beads.
From: Single-cell encoded gene silencing for high-throughput combinatorial siRNA screening
Fe3O4 beads are combined with Au nanorods and coated with PDDA, which binds siRNA, to create Knock-beads. The Au layer provides Knock-beads with photothermal responsiveness, facilitating intracellular delivery of siRNA through photoporation by 980 nm NIR laser. By utilizing a magnetic field, Knock-beads are immobilized on top of cells, enabling efficient cellular transfection of siRNAs by photoporation and single-cell encoded gene silencing in a large number of cells. Through only one-shot assay in a cell culture, more than 1300 siRNA combinations can be simultaneously analyzed to reveal the optimal siRNA therapeutic cocktail. Created in BioRender. Guo, F. (2024) BioRender.com/l65e561.
